USANA will also be offering the presentation. There will be NO phone line available to dial into, so you will need to listen to the audio through your computer. There are three rooms set up for each Web conference on the East and West Coast. 2008 H&F Presentation Kick Off - East Coast (East of Texas) Time: 6:00 p.m. MST
Live Meeting Links:
Northeast
https://www.livemeeting.com/cc/usana500/join?
Central
https://www.livemeet%20ing.com/cc/usana500/join?
Southeast
https://www.livemeet%20ing.com/cc/usana500/join?
2008 H&F Presentation Kick Off -
West CoastTime: 8:00 p.m. MST
Live Meeting links:
Northwest https://www.livemee%20ting.com/cc/usana500/join?
Centralhttps://www.livemee%20ting.com/cc/usana500/join?
Southwest https://www.livemee%20ting.com/cc/usana500/join?
We look forward to having you join one of these incredible events.
Health & Freedom '08 is sure to be a smash hit!
Showing posts with label 3H-1. Show all posts
Showing posts with label 3H-1. Show all posts
Tuesday, January 8, 2008
Monday, December 3, 2007
The JAY That Keeps Giving!
The Gift That Keeps Giving!
Upgrade to 3 Business Centers!
It was a great gift . . .
We all have similar stories! Someone . . . a friend . . . an associate . . . a family member . . . or maybe even a total stranger . . . took a deep breath to get up the courage to approach you with a gift of health and wealth: They asked you to look at the USANA opportunity!
It's the greatest gift we've ever received! It truly is the "The Gift That Keeps on Giving!" That's what Residual Income is! Like the Energizer Bunny it keeps going and going and going! For us, it been sending weekly checks for 10 and 15 years - and it doesn't stop! What a great gift!
Have you ever received a gift you didn't fully appreciate? Maybe you got the little Energizer Bunny but never found the switch that turned it on! Well, the switch is activated by reaching into the center of YOUR heart and GIVING - just like the person that shared it with you!
'tis the Season - for Giving! What a great time of the year for sharing with those we love the gift of Healthy Wealth! It's simple: ALWAYS have a Health and Freedom Newspaper or Success For Home Magazine with you and start giving them away! To everyone you meet. Some will see it as a funny little bunny but others, with your help, will find the switch! You can't control what they do - only what you do!
And now - for a limited time - USANA is offering another gift! If you or anyone in your downline are a 1 BC Associate, you have until December 28th to upgrade to a 3 BC Associate!
Login for the details. What a great way to turn on and leverage the power of your business. It's been several years since USANA has offered this opportunity - don't miss this opportunity to make sure everyone on your team is a 3 BC Associate - it can double your income! This is a limited opportunity - we must act now!
You Can Make A Difference!
Upgrade to 3 Business Centers!
It was a great gift . . .
We all have similar stories! Someone . . . a friend . . . an associate . . . a family member . . . or maybe even a total stranger . . . took a deep breath to get up the courage to approach you with a gift of health and wealth: They asked you to look at the USANA opportunity!
It's the greatest gift we've ever received! It truly is the "The Gift That Keeps on Giving!" That's what Residual Income is! Like the Energizer Bunny it keeps going and going and going! For us, it been sending weekly checks for 10 and 15 years - and it doesn't stop! What a great gift!
Have you ever received a gift you didn't fully appreciate? Maybe you got the little Energizer Bunny but never found the switch that turned it on! Well, the switch is activated by reaching into the center of YOUR heart and GIVING - just like the person that shared it with you!
'tis the Season - for Giving! What a great time of the year for sharing with those we love the gift of Healthy Wealth! It's simple: ALWAYS have a Health and Freedom Newspaper or Success For Home Magazine with you and start giving them away! To everyone you meet. Some will see it as a funny little bunny but others, with your help, will find the switch! You can't control what they do - only what you do!
And now - for a limited time - USANA is offering another gift! If you or anyone in your downline are a 1 BC Associate, you have until December 28th to upgrade to a 3 BC Associate!
Login for the details. What a great way to turn on and leverage the power of your business. It's been several years since USANA has offered this opportunity - don't miss this opportunity to make sure everyone on your team is a 3 BC Associate - it can double your income! This is a limited opportunity - we must act now!
You Can Make A Difference!
Saturday, November 24, 2007
STI WEEKLY WEBCAST PRESENTATIONS for the week Nov. 26th- Dec. 1st
WEEKLY WEBCAST PRESENTATIONS for the week Nov. 26th- Dec. 1st
US = United States Scheduled Presentations
NZ= New Zealand Scheduled Presentations
SP = Spanish Scheduled Presentations
North America (at bottom)(Anyone can join globally)
Attendance is FREE! Use this as a valuable tool to grow your USANA business fromthe comfort of your own home! Invite guests by sending them to www.successti.com and clicking on "Webcast Events" at bottom pictures or forward this by email to them.
The following meetings will broadcast internet audio directly to your computer. Please ensure that you have Windows Media Player 9 or higher installed.
(Dial connections are OK! However, we recommend you call into the conference call number for the audio portion)FIRST TIME USERS: To save time before the meeting, check your system
to make sure it is compatible with Microsoft Office Live Meeting.
For assistance, visit Live Meeting Help and Support:
http://r.office.microsoft.com/r/rlidLiveMeeting?p1=7&p2=en_US&p3=LMInfo&p4=supportSTI WEBCAST HELP PAGE FOR LOADING LIVE MEETING
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Monday Night November 26th
STI Health & Freedom Webcast Host: Mike Rhees
5:00 PM Pacific (CA)
6:00 PM Mountain (UT)
7:00 PM Central (TX)
8:00 PM Eastern (FL)
2:00 PM Auckland, NZ (on Tuesday)
(Time Zone Converter)
Click Here to Join a Few Minutes Early - Enter Your First & Last Name
Meeting ID: STI-HF-MON
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687
ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Wednesday Night November 28th
STI Health & Freedom Webcast Host : Paige Hunter
7:00 PM Pacific (CA) 8:00 PM Mountain (UT) 9:00 PM Central (TX) 10:00 PM Eastern (FL) 4:00 PM Auckland, NZ (on Thursday)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI-HF-WED
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Saturday Morning December 1st
STI Basic Training Webcast Host: Mike Rhees
8:00 AM Pacific (CA) 9:00 AM Mountain (UT) 10:00 AM Central (TX) 11:00 AM Eastern (FL) 4:00 PM London, UK 5:00 AM Auckland, NZ (on Sunday)
Click Here to Join a Few Minutes Early - Enter Your First & Last Name
Meeting ID: STI-TR-SAT
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
NZ Wednesday Night November 28th
STI Health & Freedom Webcast Hosts: Chris Folland & Fiona Jamieson-Folland
8:00 PM Auckland, NZ 11:00 PM Pacific (CA) (Tuesday) 12:00 AM Mountain (UT) 1:00 AM Central (TX) 2:00 AM Eastern (FL) 7:00 AM London, UK
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI- NZ- HF Role: Attendee
CONFERENCE CALL +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
SP Wednesday Night November
28th STI SPANISH Health & Freedom
Webcast Host: Valdemar Garza
7:00 PM Pacific (CA) 8:00 PM Mountain (UT) 9:00 PM Central (TX) & Mexico (South, Central, Eastern observing DST time) 10:00 PM Eastern (FL) 4:00 PM Auckland, NZ (on Thursday)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: R52NPM Role: Attendee
(No conference call number, audio broadcast through computer speakers only)
Haga clic aquí para participar en Live Meeting
Dirección URL de la reunión: https://www.livemeeting.com/cc/usana500/join
Id. de la reunión: R52NPM
Función: Asistente
Esta reunión se difundirá por audio a través de Internet directamente en su equipo. Asegúrese de que tiene instalado Reproductor de Windows Media 9 o superior.
USUARIOS QUE UTILIZAN EL SISTEMA POR PRIMERA VEZ: para ahorrar tiempo antes de la reunión, compruebe el sistema para asegurarse de que es compatible con Microsoft Office Live Meeting.
Esta invitación de Live Meeting es personal; no se debe reenviar.
Para obtener ayuda, visite las secciones de Ayuda y Soporte técnico de Live Meeting: http://r.office.microsoft.com/r/rlidLiveMeeting?p1=7&p2=es_ES&p3=LMInfo&p4=support
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US = United States Scheduled Presentations
NZ= New Zealand Scheduled Presentations
SP = Spanish Scheduled Presentations
North America (at bottom)(Anyone can join globally)
Attendance is FREE! Use this as a valuable tool to grow your USANA business fromthe comfort of your own home! Invite guests by sending them to www.successti.com and clicking on "Webcast Events" at bottom pictures or forward this by email to them.
The following meetings will broadcast internet audio directly to your computer. Please ensure that you have Windows Media Player 9 or higher installed.
(Dial connections are OK! However, we recommend you call into the conference call number for the audio portion)FIRST TIME USERS: To save time before the meeting, check your system
to make sure it is compatible with Microsoft Office Live Meeting.
For assistance, visit Live Meeting Help and Support:
http://r.office.microsoft.com/r/rlidLiveMeeting?p1=7&p2=en_US&p3=LMInfo&p4=supportSTI WEBCAST HELP PAGE FOR LOADING LIVE MEETING
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Monday Night November 26th
STI Health & Freedom Webcast Host: Mike Rhees
5:00 PM Pacific (CA)
6:00 PM Mountain (UT)
7:00 PM Central (TX)
8:00 PM Eastern (FL)
2:00 PM Auckland, NZ (on Tuesday)
(Time Zone Converter)
Click Here to Join a Few Minutes Early - Enter Your First & Last Name
Meeting ID: STI-HF-MON
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687
ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Wednesday Night November 28th
STI Health & Freedom Webcast Host : Paige Hunter
7:00 PM Pacific (CA) 8:00 PM Mountain (UT) 9:00 PM Central (TX) 10:00 PM Eastern (FL) 4:00 PM Auckland, NZ (on Thursday)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI-HF-WED
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Saturday Morning December 1st
STI Basic Training Webcast Host: Mike Rhees
8:00 AM Pacific (CA) 9:00 AM Mountain (UT) 10:00 AM Central (TX) 11:00 AM Eastern (FL) 4:00 PM London, UK 5:00 AM Auckland, NZ (on Sunday)
Click Here to Join a Few Minutes Early - Enter Your First & Last Name
Meeting ID: STI-TR-SAT
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
NZ Wednesday Night November 28th
STI Health & Freedom Webcast Hosts: Chris Folland & Fiona Jamieson-Folland
8:00 PM Auckland, NZ 11:00 PM Pacific (CA) (Tuesday) 12:00 AM Mountain (UT) 1:00 AM Central (TX) 2:00 AM Eastern (FL) 7:00 AM London, UK
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI- NZ- HF Role: Attendee
CONFERENCE CALL +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
SP Wednesday Night November
28th STI SPANISH Health & Freedom
Webcast Host: Valdemar Garza
7:00 PM Pacific (CA) 8:00 PM Mountain (UT) 9:00 PM Central (TX) & Mexico (South, Central, Eastern observing DST time) 10:00 PM Eastern (FL) 4:00 PM Auckland, NZ (on Thursday)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: R52NPM Role: Attendee
(No conference call number, audio broadcast through computer speakers only)
Haga clic aquí para participar en Live Meeting
Dirección URL de la reunión: https://www.livemeeting.com/cc/usana500/join
Id. de la reunión: R52NPM
Función: Asistente
Esta reunión se difundirá por audio a través de Internet directamente en su equipo. Asegúrese de que tiene instalado Reproductor de Windows Media 9 o superior.
USUARIOS QUE UTILIZAN EL SISTEMA POR PRIMERA VEZ: para ahorrar tiempo antes de la reunión, compruebe el sistema para asegurarse de que es compatible con Microsoft Office Live Meeting.
Esta invitación de Live Meeting es personal; no se debe reenviar.
Para obtener ayuda, visite las secciones de Ayuda y Soporte técnico de Live Meeting: http://r.office.microsoft.com/r/rlidLiveMeeting?p1=7&p2=es_ES&p3=LMInfo&p4=support
-+-----+-----+-----+-----+-----+-----+-----+-----+-
Sunday, November 18, 2007
CHECK THIS OTHER USANA BLOG OUT
SEARCHING THROUGH THE BLOG I CAME ACROSS THIS OTHER BLOG
CHECK IT OUT:
http://www.usanachoice.blogspot.com/
http://www.safeliving.usana.com/
CHECK IT OUT:
http://www.usanachoice.blogspot.com/
http://www.safeliving.usana.com/
Vitamin D levels inadequate in half of women treated with osteoporosis drugs
Vitamin D levels inadequate in half of women treated with osteoporosis drugs
Despite efforts to increase knowledge and emphasis on osteoporosis prevention and treatment, research indicates that many women treated for osteoporosis have low levels of vitamin D, a nutrient necessary for adequate bone mineralization.A recent study involved 1,536 postmenopausal women from 61 study sites who had been taking medications for the treatment of osteoporosis for a minimum of three months. Participants were allowed to have used vitamin D supplements as long as the dosage had remained consistent.
Overall, 52 percent of the women had vitamin D levels considered inadequate. Sixty-three percent of women who reported a supplemental intake of 400 IU's or less had inadequate vitamin D levels compared to 45 percent of those whose intake was 400 IU's or greater.
Lower levels were also associated with the lack of physician counseling regarding the importance of vitamin D in bone health. The results of this study emphasize the need for greater education of the public and physicians regarding the significance of vitamin D status in the care of postmenopausal women with osteoporosis.
http://www.safeliving.usana.com
Despite efforts to increase knowledge and emphasis on osteoporosis prevention and treatment, research indicates that many women treated for osteoporosis have low levels of vitamin D, a nutrient necessary for adequate bone mineralization.A recent study involved 1,536 postmenopausal women from 61 study sites who had been taking medications for the treatment of osteoporosis for a minimum of three months. Participants were allowed to have used vitamin D supplements as long as the dosage had remained consistent.
Overall, 52 percent of the women had vitamin D levels considered inadequate. Sixty-three percent of women who reported a supplemental intake of 400 IU's or less had inadequate vitamin D levels compared to 45 percent of those whose intake was 400 IU's or greater.
Lower levels were also associated with the lack of physician counseling regarding the importance of vitamin D in bone health. The results of this study emphasize the need for greater education of the public and physicians regarding the significance of vitamin D status in the care of postmenopausal women with osteoporosis.
http://www.safeliving.usana.com
Business Building During the Holidays
Business Building During the Holidays
By Pete Zdanis
The holiday season is fast approaching, and with it can come a whole set of new obstacles for the uninformed network marketer.
The obstacles:
Uninformed Network Marketer Mentality: “I’m going to take some time off during the holidays, and then start building my business again after the New Year.”
Uninformed Prospects Mentality: “Your business really sounds interesting to me, but why don’t you get back to me after the New Year, and we can discuss it then.”
How can we address these obstacles? Let’s start with the uninformed network marketer mentality:
Momentum is a very important part of our business. Momentum is simply maintaining a steady pace at anything. Once you lose it, it requires a lot of effort to get it back.
Think about driving your car down the highway at a steady 65 miles per hour. You are moving toward your destination and making good progress. However, when you pull off to the side of the road and stop, you are losing time, and it will require more time and energy (in this case, gasoline) to get back on the highway and accelerate back up to your previous cruising speed to get where you want to go.
The same is true of your business. When you stop making approaches and followups, you are no longer moving toward your business goals. And, the longer you take “time off”, the more time you lose and the harder it will be for you to get back to your previous business building pace. You will need to start making approaches from scratch, and you will have lost many of your prospects due to lack of followup.
Let’s say that you follow the tried and true method laid out in the “Power Of Two”. If you stop building your business from now until, say, January 2, you will have missed out on 98 approaches, 14 presentations, and at least 2 or 3 enrollments. Can you afford that? I know that we can’t.
And don’t fall into the trap of thinking that you don’t want to “bother” people with something like USANA during the holiday season. As you know, this is the season of giving, and the USANA products and business truly are gifts which we offer to our prospects, and it is up to them do decide if they choose to accept our gift.
If you don’t believe that USANA is a gift, then you need to re-think your belief level and business motives. Remember, building a USANA business has nothing to do with you, and has everything to do with your customers and prospects. Let me repeat that: Building a USANA business has nothing to do with you, and has everything to do with your customers and prospects. When you help other people meet a need or solve a problem, abundance will definitely come your way – for you AND your team members.
If you are not comfortable with that concept, try a simple exercise the next time you make an approach or presentation. Instead of thinking “What will signing this person up as an Associate or Preferred Customer do for my business?”, approach it from the standpoint of “How can my gift of USANA help this person?” You will be truly amazed at the difference in your results by making this simple shift in your thinking.
You see, so many people get things backwards when trying to build their USANA business, and that’s what leads to problems and failure. I know when I started out and was talking to a prospect, the foremost thoughts in my mind were where I was going to place this person when they signed up, and how much it would help my business. Now admit it, you’ve probably had the same thoughts, and maybe you still do from time to time. But, once I focused on trying to figure out how my gift could help my prospects, everything changed for the better for everyone, including me.
Now let’s talk about the uninformed prospect:
If a prospect tries to put you off until after the holidays, first of all tell them that you appreciate their concerns and respect their time, but that you just want to make two points with them before they make a final decision to wait until January.
Tell them that most people feel the same was as they do, and want to wait until after the holidays. So, what happens is that January and February are very strong business building months because people are looking to make a new start and improve their health and/or financial situation. By getting their USANA business set up now, your prospect can benefit from all of this activity instead of just being a part of it.
A major benefit of having a USANA business can be the tax advantages it offers. By getting their business set up now, they can potentially benefit from tax savings when they file their return in a few months, rather than putting those benefits off until they file their tax return in 2009.
Tell them that you respect whatever decision they make, but that you just wanted to make sure that they were aware of those two major benefits.
Remember, building a USANA business has nothing to do with you, and has everything to do with your customers and prospects.
By Pete Zdanis
The holiday season is fast approaching, and with it can come a whole set of new obstacles for the uninformed network marketer.
The obstacles:
Uninformed Network Marketer Mentality: “I’m going to take some time off during the holidays, and then start building my business again after the New Year.”
Uninformed Prospects Mentality: “Your business really sounds interesting to me, but why don’t you get back to me after the New Year, and we can discuss it then.”
How can we address these obstacles? Let’s start with the uninformed network marketer mentality:
Momentum is a very important part of our business. Momentum is simply maintaining a steady pace at anything. Once you lose it, it requires a lot of effort to get it back.
Think about driving your car down the highway at a steady 65 miles per hour. You are moving toward your destination and making good progress. However, when you pull off to the side of the road and stop, you are losing time, and it will require more time and energy (in this case, gasoline) to get back on the highway and accelerate back up to your previous cruising speed to get where you want to go.
The same is true of your business. When you stop making approaches and followups, you are no longer moving toward your business goals. And, the longer you take “time off”, the more time you lose and the harder it will be for you to get back to your previous business building pace. You will need to start making approaches from scratch, and you will have lost many of your prospects due to lack of followup.
Let’s say that you follow the tried and true method laid out in the “Power Of Two”. If you stop building your business from now until, say, January 2, you will have missed out on 98 approaches, 14 presentations, and at least 2 or 3 enrollments. Can you afford that? I know that we can’t.
And don’t fall into the trap of thinking that you don’t want to “bother” people with something like USANA during the holiday season. As you know, this is the season of giving, and the USANA products and business truly are gifts which we offer to our prospects, and it is up to them do decide if they choose to accept our gift.
If you don’t believe that USANA is a gift, then you need to re-think your belief level and business motives. Remember, building a USANA business has nothing to do with you, and has everything to do with your customers and prospects. Let me repeat that: Building a USANA business has nothing to do with you, and has everything to do with your customers and prospects. When you help other people meet a need or solve a problem, abundance will definitely come your way – for you AND your team members.
If you are not comfortable with that concept, try a simple exercise the next time you make an approach or presentation. Instead of thinking “What will signing this person up as an Associate or Preferred Customer do for my business?”, approach it from the standpoint of “How can my gift of USANA help this person?” You will be truly amazed at the difference in your results by making this simple shift in your thinking.
You see, so many people get things backwards when trying to build their USANA business, and that’s what leads to problems and failure. I know when I started out and was talking to a prospect, the foremost thoughts in my mind were where I was going to place this person when they signed up, and how much it would help my business. Now admit it, you’ve probably had the same thoughts, and maybe you still do from time to time. But, once I focused on trying to figure out how my gift could help my prospects, everything changed for the better for everyone, including me.
Now let’s talk about the uninformed prospect:
If a prospect tries to put you off until after the holidays, first of all tell them that you appreciate their concerns and respect their time, but that you just want to make two points with them before they make a final decision to wait until January.
Tell them that most people feel the same was as they do, and want to wait until after the holidays. So, what happens is that January and February are very strong business building months because people are looking to make a new start and improve their health and/or financial situation. By getting their USANA business set up now, your prospect can benefit from all of this activity instead of just being a part of it.
A major benefit of having a USANA business can be the tax advantages it offers. By getting their business set up now, they can potentially benefit from tax savings when they file their return in a few months, rather than putting those benefits off until they file their tax return in 2009.
Tell them that you respect whatever decision they make, but that you just wanted to make sure that they were aware of those two major benefits.
Remember, building a USANA business has nothing to do with you, and has everything to do with your customers and prospects.
Health & Freedom '08 Presentation
Inviting someone to a Health & Freedom '08 Presentation can be the hardest part of building your business.
Use our invitation ideas and join a training Web conference on inviting on Monday, November 19 with expert Tim Sales.
Tim Sales is a master network marketer and while working his former network marketing business, earned $150,000 a month and had over 56,000 people in his organization.
Tim's successful experience and knowledge make him one of the most sought after advisors, speakers, trainers, and creators of content in the industry.
We are pleased to welcome Tim on this Web conference! Please join us!
DetailsNovember 19, 2007
How to invite with Tim SalesTime: 6:00 p.m.
MSTCall-in Number: 641-594-7500PIN: 641342
Live Meeting link:
https://www.livemeeting.com/cc/usana500/join?id=HFTRAIN1&role=attend (West Coast)
https://www.livemeeting.com/cc/usana500/join?id=HFTRAIN2&role=attend (East Coast)
See you on the webcast!Chris LopezTo unsubscribe or change subscriber options visit:
https://m247.infusionsoft.com/rem/x186a0/jason_s_kilgore!hotmail.com/544a37700/
Integritas Group
PO Box 025250
# 41757
Miami, FL 33102
Delivered By Infusion Software
http://www.infusionsoft.com
Use our invitation ideas and join a training Web conference on inviting on Monday, November 19 with expert Tim Sales.
Tim Sales is a master network marketer and while working his former network marketing business, earned $150,000 a month and had over 56,000 people in his organization.
Tim's successful experience and knowledge make him one of the most sought after advisors, speakers, trainers, and creators of content in the industry.
We are pleased to welcome Tim on this Web conference! Please join us!
DetailsNovember 19, 2007
How to invite with Tim SalesTime: 6:00 p.m.
MSTCall-in Number: 641-594-7500PIN: 641342
Live Meeting link:
https://www.livemeeting.com/cc/usana500/join?id=HFTRAIN1&role=attend (West Coast)
https://www.livemeeting.com/cc/usana500/join?id=HFTRAIN2&role=attend (East Coast)
See you on the webcast!Chris LopezTo unsubscribe or change subscriber options visit:
https://m247.infusionsoft.com/rem/x186a0/jason_s_kilgore!hotmail.com/544a37700/
Integritas Group
PO Box 025250
# 41757
Miami, FL 33102
Delivered By Infusion Software
http://www.infusionsoft.com
STI Health & Freedom Webcasts
US Monday November 19th
Night STI Health & Freedom Webcast Host: Jared Crebs
(please note 1 hour time change to promote for our USANA web cast at 6:00mst)
6:00 PM Pacific (CA)
7:00 PM Mountain (UT)
8:00 PM Central (TX)
9:00 PM Eastern (FL)
2:00 PM Auckland, NZ (on Tuesday)
(Time Zone Converter)Click Here to Join a Few Minutes Early - Enter Your First & Last Name
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI-HF-MON
Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Wednesday Night
STI Health & Freedom Webcast NO WEB CAST THANKSGIVING
7:00 PM Pacific (CA)
8:00 PM Mountain (UT)
9:00 PM Central (TX)
10:00 PM Eastern (FL)
3:00 PM Auckland, NZ (on Thursday)
(Time Zone Converter)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI-HF-WED Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
US Saturday Morning
STI Basic Training Webcast
8:00 AM Pacific (CA)
9:00 AM Mountain (UT)
10:00 AM Central (TX)
11:00 AM Eastern (FL)
4:00 PM London, UK
4:00 AM Auckland, NZ (on Sunday)
(Time Zone Converter)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI-TR-SAT Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951
NZ Wednesday Night
STI Health & Freedom Webcast
8:00 PM Auckland, NZ
12:00 AM Pacific (CA) (Tuesday)
1:00 AM Mountain (UT)
2:00 AM Central (TX)
3:00 AM Eastern (FL)
8:00 AM London, UK
(Time Zone Converter)
https://www.livemeeting.com/cc/usana500/join
Meeting ID: STI- NZ- HF Role: Attendee
CONFERENCE CALL NUMBER +1 (218) 936-7687 ID: 283951#
-+-----+-----+-----+-----+-----+-----+-----+-----+-
Night STI Health & Freedom Webcast Host: Jared Crebs
(please note 1 hour time change to promote for our USANA web cast at 6:00mst)
6:00 PM Pacific (CA)
7:00 PM Mountain (UT)
8:00 PM Central (TX)
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2:00 PM Auckland, NZ (on Tuesday)
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Saturday, November 3, 2007
Antioxidants and Phase 2 Enzymes in Cardiomyocytes
Antioxidants and Phase 2 Enzymes in Cardiomyocytes:
Chemical Inducibility and Chemoprotection Against Oxidant and Simulated Ischemia-Reperfusion Injury Zhuoxiao Cao*,1, Hong Zhu*, Li Zhang*, Xue Zhao, Jay L. Zweier* and Yunbo Li*,2
* Davis Heart and Lung Research Institute, Department of Internal Medicine and Division of Cardiovascular Medicine, The Ohio State University Medical Center, Columbus, Ohio 43210; and Department of Cardiology, Changzheng Hospital, The Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China
The increasing recognition of the role for oxidative stress in cardiac disorders has led to extensive investigation on the protection by exogenous antioxidants against oxidative cardiac injury. On the other hand, another strategy for protecting against oxidative cardiac injury may be through upregulation of the endogenous antioxidants and phase 2 enzymes in the myocardium by chemical inducers. However, our current understanding of the chemical inducibility of cardiac cellular antioxidants and phase 2 enzymes is very limited.
In this study, using rat cardiac H9c2 cells we have characterized the concentration- and time-dependent induction of cellular antioxidants and phase 2 enzymes by 3H-1,2-dithiole-3-thione (D3T), and the resultant chemoprotective effects on oxidative cardiac cell injury. Incubation of H9c2 cells with D3T resulted in a marked concentration- and time-dependent induction of a number of cellular antioxidants and phase 2 enzymes, including catalase, reduced glutathione (GSH), GSH peroxidase, glutathione reductase (GR), GSH S-transferase (GST), and NAD(P)H:quinone oxidoreductase-1 (NQO1). D3T treatment of H9c2 cells also caused an increase in mRNA expression of catalase, -glutamylcysteine ligase catalytic subunit, GR, GSTA1, M1 and P1, and NQO1.
Moreover, both mRNA and protein expression of Nrf2 were induced in D3T-treated cells. D3T pretreatment led to a marked protection against H9c2 cell injury elicited by various oxidants and simulated ischemia-reperfusion. D3T pretreatment also resulted in decreased intracellular accumulation of reactive oxygen in H9c2 cells after exposure to the oxidants as well as simulated ischemia-reperfusion. This study demonstrates that a series of endogenous antioxidants and phase 2 enzymes in H9c2 cells can be induced by D3T in a concentration- and time-dependent fashion, and that the D3T-upregulated cellular defenses are accompanied by a markedly increased resistance to oxidative cardiac cell injury.
Key Words: cardiac cells • 3H-1,2-dithiole-3-thione • antioxidants • oxidants • simulated ischemia-reperfusion • cytotoxicity
Chemical Inducibility and Chemoprotection Against Oxidant and Simulated Ischemia-Reperfusion Injury Zhuoxiao Cao*,1, Hong Zhu*, Li Zhang*, Xue Zhao, Jay L. Zweier* and Yunbo Li*,2
* Davis Heart and Lung Research Institute, Department of Internal Medicine and Division of Cardiovascular Medicine, The Ohio State University Medical Center, Columbus, Ohio 43210; and Department of Cardiology, Changzheng Hospital, The Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China
The increasing recognition of the role for oxidative stress in cardiac disorders has led to extensive investigation on the protection by exogenous antioxidants against oxidative cardiac injury. On the other hand, another strategy for protecting against oxidative cardiac injury may be through upregulation of the endogenous antioxidants and phase 2 enzymes in the myocardium by chemical inducers. However, our current understanding of the chemical inducibility of cardiac cellular antioxidants and phase 2 enzymes is very limited.
In this study, using rat cardiac H9c2 cells we have characterized the concentration- and time-dependent induction of cellular antioxidants and phase 2 enzymes by 3H-1,2-dithiole-3-thione (D3T), and the resultant chemoprotective effects on oxidative cardiac cell injury. Incubation of H9c2 cells with D3T resulted in a marked concentration- and time-dependent induction of a number of cellular antioxidants and phase 2 enzymes, including catalase, reduced glutathione (GSH), GSH peroxidase, glutathione reductase (GR), GSH S-transferase (GST), and NAD(P)H:quinone oxidoreductase-1 (NQO1). D3T treatment of H9c2 cells also caused an increase in mRNA expression of catalase, -glutamylcysteine ligase catalytic subunit, GR, GSTA1, M1 and P1, and NQO1.
Moreover, both mRNA and protein expression of Nrf2 were induced in D3T-treated cells. D3T pretreatment led to a marked protection against H9c2 cell injury elicited by various oxidants and simulated ischemia-reperfusion. D3T pretreatment also resulted in decreased intracellular accumulation of reactive oxygen in H9c2 cells after exposure to the oxidants as well as simulated ischemia-reperfusion. This study demonstrates that a series of endogenous antioxidants and phase 2 enzymes in H9c2 cells can be induced by D3T in a concentration- and time-dependent fashion, and that the D3T-upregulated cellular defenses are accompanied by a markedly increased resistance to oxidative cardiac cell injury.
Key Words: cardiac cells • 3H-1,2-dithiole-3-thione • antioxidants • oxidants • simulated ischemia-reperfusion • cytotoxicity
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